Part:BBa_K5286003

This part contains Hypsibius dujardini's heat soluble protein CAHS107838 coding sequence (BBa_K5286000), and a 10XHisTag with a single stop codon, which under the expression of an inducible promoter allows for protein purification according to the protocol by Boothby et al.(2017):

"Cells were then lysed by heat shock at 95°C for 15 min. Lysates were cooled at room temperature for 30 min. Insoluble components were removed by centrifugation at 20,000g and 10°C for 30 min."

The protein can then be further purified with a HisTag purification kit.

Sequence and features

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal prefix found in sequence at 702
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 702
  Illegal NotI site found at 708
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 702
  Illegal XhoI site found at 559
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found in sequence at 702
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found in sequence at 702
  Illegal AgeI site found at 761
  
• 1000
  COMPATIBLE WITH RFC[1000]